Rpl22^tm1.2Psam
Targeted Allele Detail

Summary

• Symbol: Rpl22^tm1.2Psam
• Name: ribosomal protein L22; targeted mutation 1.2, Paul S Amieux
• MGI ID: MGI:4355968
• Gene: Rpl22 Location: Chr4:152410199-152418528 bp, + strand Genetic Position: Chr4, 83.03 cM, cytoband E2
• Alliance: Rpl22^tm1.2Psam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151962
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: Mice carrying the Rpl22^tm1.1Psam allele were crossed with Mox2-cre mice to remove the endogenous exon 4 from the germline. This allowed transcription of the knocked-in exon 4 that contains sequence encoding three hemagglutinin eptiopes upstream of the stop codon. Immunoblot analysis confirmed in homozygote tissue extracts that the mutant protein was expressed at levels similar to endogenous protein in controls. Mutant protein expression effectively "tags" ribosomal complexes with the HA epitope allowing for their isolation. (J:151962)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rpl22 Mutation: 46 strains or lines available

References

• Original: J:151962 Sanz E, et al., Cell-type-specific isolation of ribosome-associated mRNA from complex tissues. Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13939-44
• All: 2 reference(s)