Tg(CD2-TcraAc1-9,-TcrbAc1-9)4Kio
Transgene Detail

Summary

• Symbol: Tg(CD2-TcraAc1-9,-TcrbAc1-9)4Kio
• Name: transgene insertion 4, Dimitris Kioussis
• MGI ID: MGI:4354587
• Synonyms: Tg4
• Transgene: Tg(CD2-TcraAc1-9,-TcrbAc1-9)4Kio Location: unknown
• Alliance: Tg(CD2-TcraAc1-9,-TcrbAc1-9)4Kio page

Transgene origin

• Strain of Origin: (CBA x C57BL/10)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CD2-TcraAc1-9,-TcrbAc1-9)4Kio expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: The TCR alpha and beta transgenic constructs derived from a Ac1-9-specific T cell hybridoma were generated by inserting cDNAs containing the leader and coding sequences into an expression cassette containing the human CD2 promoter. RNA splice sites and a poly(A) signal sequence were introduced by ligating a genomic segment of the human beta-globin gene 3' to the TCR sequences. Transgenic mice express the Vbeta8.2 TCR specific for myelin basic protein (MBP) epitope Ac1-9 in the context of I-A^u in T cells. In addition to line 4, line Tg3 was also created. (J:109162)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

References

• Original: J:109162 Liu GY, et al., Low avidity recognition of self-antigen by T cells permits escape from central tolerance. Immunity. 1995 Oct;3(4):407-15
• All: 22 reference(s)