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Targeted Allele Detail
Symbol: Gucy1a1tm1.1Brou
Name: guanylate cyclase 1, soluble, alpha 1; targeted mutation 1.1, Peter Brouckaert
MGI ID: MGI:4354143
Synonyms: sGCalph1-
Gene: Gucy1a1  Location: Chr3:81999734-82053096 bp, - strand  Genetic Position: Chr3, 35.97 cM
Alliance: Gucy1a1tm1.1Brou page
Germline Transmission:  Earliest citation of germline transmission: J:151161
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsExon 6 of Gucy1a3 gene which codes for a conserved portion of the catalytic domain was flanked by a loxP and a loxP-neo-loxP cassette. The exon 6 and neo cassette was removed in ES cells by transient cre expression leaving a single loxP in place. Northern blot and RT-PCR confirmed excision of exon 6 in the transcript; however, immunoblot revealed a smaller mutant 59-kDa protein in homozygous mice. Mutant protein was determined to be inactive, thus it is functional null allele. (J:151161)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Mice Carrying this Mutation: 11 assay results
In Structures Affected by this Mutation: 9 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gucy1a1 Mutation:  27 strains or lines available
Original:  J:113106 Cauwels A, et al., Anaphylactic shock depends on PI3K and eNOS-derived NO. J Clin Invest. 2006 Aug;116(8):2244-51
All:  16 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
MGI 6.22
The Jackson Laboratory