Tert^tm2.1Sear
Targeted Allele Detail

Summary

• Symbol: Tert^tm2.1Sear
• Name: telomerase reverse transcriptase; targeted mutation 2.1, Steven E Artandi
• MGI ID: MGI:4352569
• Synonyms: Tert^AFH
• Gene: Tert Location: Chr13:73775030-73797962 bp, + strand Genetic Position: Chr13, 40.12 cM
• Alliance: Tert^tm2.1Sear page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150343
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A compound epitope tag comprising a Staph protein A sequence, a Flag epitope and a triple HA epitope was inserted in frame upstream of the initiating methionine codon in exon 1. A floxed neomycin cassette was inserted downstream of exon 1. Targeting occurred in ES cells with a knock-in of tamoxifin-inducible cre recombinase. Tamoxifen incubation led to the removal of the neomycin selection cassette in correctly targeted ES cells. Telomerase activity was equivalent between homozygotes and controls as determined by telomerase activity from small intestine extracts. (J:150343)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tert Mutation: 52 strains or lines available

Notes

ES cells contain a cre/ERT2 fusion gene knocked into the Gt(ROSA)26Sor locus.

References

• Original: J:150343 Park JI, et al., Telomerase modulates Wnt signalling by association with target gene chromatin. Nature. 2009 Jul 2;460(7251):66-72
• All: 1 reference(s)