Plekhg5^{Gt(E277F07)Wrst}
Gene trapped Allele Detail

Summary

• Symbol: Plekhg5^{Gt(E277F07)Wrst}
• Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 5; gene trap E277F07, German Gene Trap Consortium
• MGI ID: MGI:4352190
• Gene: Plekhg5 Location: Chr4:152156955-152199857 bp, + strand Genetic Position: Chr4, 82.86 cM
• Alliance: Plekhg5^{Gt(E277F07)Wrst} page

Mutation origin

• Mutant Cell Line: E277F07
• Germline Transmission: Earliest citation of germline transmission: J:252982
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: reFROSAbgeo+1s
• Mutation details: The gene trap cassette inserted into intron 4. RT-PCR analysis showed that the mutation eliminated normal mRNA expression of all four known isoforms of the gene. A fusion protein consisting of the first 28 amino acids fused to beta-GEO is expressed from this allele. No wild-type protein is detectable in immunostaining experiments. (J:252982)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  5SE277F07	FI851321 ; (MGI Seq Detail)	5'Splinkerette PCR	Chr4:152183119-152183445 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  52 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plekhg5 Mutation: 97 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)