Akap13^{Gt(AG0213)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Akap13^{Gt(AG0213)Wtsi}
• Name: A kinase anchor protein 13; gene trap AG0213, Wellcome Trust Sanger Institute
• MGI ID: MGI:4345910
• Synonyms: deltaBrx
• Gene: Akap13 Location: Chr7:75105282-75404357 bp, + strand Genetic Position: Chr7, 43.76 cM, cytoband D1
• Alliance: Akap13^{Gt(AG0213)Wtsi} page

Mutation origin

• Mutant Cell Line: AG0213
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: The beta-geo gene trap was inserted into intron 8. This mutation creates a fusion of the AKAP-Lbc and Brx isoforms with beta-geo that disrupts the Rho-guanine nucleotide exchange factor (GEF) and protein kinase D (PKD) binding domains for these two isoforms. However, the Lbc isoform is normally expressed. Quantitative PCR analysis of neonatal heart and lung RNA revealed that expression of the Brx-9 exon-exon junction is reduced in heterozygous and completely eliminated in homozygous mutant mice. Expression of the E37-38 exon-exon junction is reduced in a dose-dependent manner while expression of the E4-5 exon-exon junction is unaffected. (J:231257)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AG0213	CL525698 ; (MGI Seq Detail)	5' RACE	Chr7:75292971-75293211 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  59 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Akap13^{Gt(AG0213)Wtsi}, Akap13^{Gt(CSJ288)Byg}, and Akap13^{Gt(CSJ306)Byg} alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Akap13 Mutation: 178 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)