Mcc^{Gt(W197F04)Wrst}
Gene trapped Allele Detail

Summary

• Symbol: Mcc^{Gt(W197F04)Wrst}
• Name: mutated in colorectal cancers; gene trap W197F04, German Gene Trap Consortium
• MGI ID: MGI:4335844
• Synonyms: Mcc^{Gt(W197F04)GGTC}, Mcc^W
• Gene: Mcc Location: Chr18:44558127-44945249 bp, - strand Genetic Position: Chr18, 23.74 cM
• Alliance: Mcc^{Gt(W197F04)Wrst} page

Mutation origin

• Mutant Cell Line: W197F04
• Germline Transmission: Earliest citation of germline transmission: J:177409
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: ROSAbetageo
• Mutation details: The gene trap vector inserted 96.2 kb 3' of exon 3. Western blot analysis confirmed the absence of expression of isoform 1 but isoform 2 of the protein is expressed at wild-type levels. Very little beta-galactosidase activity is detected in heterozygotes. (J:177409)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  W197F04	CL213553 ; (MGI Seq Detail)	5' RACE	Chr18:44857531-44857671 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  18 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

19 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcc Mutation: 58 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)