Asap1^{Gt(RRS873)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Asap1^{Gt(RRS873)Byg}
• Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain1; gene trap RRS873, BayGenomics
• MGI ID: MGI:4332097
• Synonyms: Asap1^GT
• Gene: Asap1 Location: Chr15:63958706-64254768 bp, - strand Genetic Position: Chr15, 28.55 cM, cytoband D3
• Alliance: Asap1^{Gt(RRS873)Byg} page

Mutation origin

• Mutant Cell Line: RRS873
• Germline Transmission: Earliest citation of germline transmission: J:277387
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: A gene trap vector (pGT0Lxf) containing a splice-acceptor sequence upstream of a beta-geo reporter gene and a polyA tail was inserted into intron 2 between nucleotides 64,308,443 and 64,316,218, resulting in deletion of the corresponding part of the gene including exon 3 (nt 64,312,349-64,312,475). Semi-quantitative RT-PCR and Western blot analysis confirmed the absence of mRNA and protein expression in E11.5 homozygous mutant embryos, while X-Gal staining revealed that expression of lacZ is detectable in E10.5 heterozygous and homozygous mutant embryos. All 31 exons 3' to the insertion site of the vector are not expressed. (J:277387)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRS873	CG887063 ; (MGI Seq Detail)	5' RACE	Chr15:64221677-64254625 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  16 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	60 assay results
In Structures Affected by this Mutation:	10 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Asap1^{Gt(RRS873)Byg}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Asap1 Mutation: 60 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)