Pias1^{Gt(RRQ113)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Pias1^{Gt(RRQ113)Byg}
• Name: protein inhibitor of activated STAT 1; gene trap RRQ113, BayGenomics
• MGI ID: MGI:4330151
• Gene: Pias1 Location: Chr9:62785648-62888161 bp, - strand Genetic Position: Chr9, 33.91 cM
• Alliance: Pias1^{Gt(RRQ113)Byg} page

Mutation origin

• Mutant Cell Line: RRQ113
• Germline Transmission: Earliest citation of germline transmission: J:234118
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A gene trap construct inserted after exon 1, introducing a polyadenylation site and a premature stop codon. RT-PCR and immunoblotting experiments confirmed the loss of wild-type transcript and protein, respectively. (J:234118)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRQ113	CG784310 ; (MGI Seq Detail)	5' RACE	Chr9:62888131-62888171 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  13 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pias1 Mutation: 44 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)