Rnf20^{Gt(RRJ249)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Rnf20^{Gt(RRJ249)Byg}
• Name: ring finger protein 20; gene trap RRJ249, BayGenomics
• MGI ID: MGI:4129198
• Synonyms: RNF20^-
• Gene: Rnf20 Location: Chr4:49632006-49656887 bp, + strand Genetic Position: Chr4, 26.69 cM
• Alliance: Rnf20^{Gt(RRJ249)Byg} page

Mutation origin

• Mutant Cell Line: RRJ249
• Germline Transmission: Earliest citation of germline transmission: J:268221
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: A gene trap vector inserted between exons 17 and 18. The predicted protein product is expected to lack the catalytic RING domain but retain most of the remaining protein. Western blot analysis of lung homogenates from heterozygous mutant mice failed to detect the truncated protein, suggesting its intrinsic instability. (J:268221)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRJ249	CC326118 ; (MGI Seq Detail)	5' RACE	Chr4:49651485-49653273 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnf20 Mutation: 44 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)