Kcmf1^Gt(XE080)Byg
Gene trapped Allele Detail

Summary

• Symbol: Kcmf1^Gt(XE080)Byg
• Name: potassium channel modulatory factor 1; gene trap XE080, BayGenomics
• MGI ID: MGI:4124894
• Synonyms: XE-080^LacZ
• Gene: Kcmf1 Location: Chr6:72818097-72876962 bp, - strand Genetic Position: Chr6, 32.3 cM, cytoband C3
• Alliance: Kcmf1^Gt(XE080)Byg page

Mutation origin

• Mutant Cell Line: XE080
• Germline Transmission: Earliest citation of germline transmission: J:162125
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted between exon 1 and 2. Expression of the fusion construct was confirmed by western blot analysis. RT-PCR and western blot analysis confirmed a reduction in transcript and protein expression, respectively, in the liver. (J:162125)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE080	CC178232 ; (MGI Seq Detail)	5' RACE	Chr6:72876598-72876688 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  161 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcmf1 Mutation: 112 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)