Dnajb9^{Gt(KST256)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Dnajb9^{Gt(KST256)Byg}
• Name: DnaJ heat shock protein family (Hsp40) member B9; gene trap KST256, BayGenomics
• MGI ID: MGI:4124484
• Synonyms: ERdj4^GT
• Gene: Dnajb9 Location: Chr12:44252680-44256851 bp, - strand Genetic Position: Chr12, 20.71 cM
• Alliance: Dnajb9^{Gt(KST256)Byg} page

Mutation origin

• Mutant Cell Line: KST256
• Germline Transmission: Earliest citation of germline transmission: J:226674
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1TMpfs Vector Type: secretory trap
• Mutation details: The gene trap cassette inserted between adenosine 1151 and guanosine 1152 within intron 1 of the gene, resulting in splicing of exon 1 with the GT cassette and causing premature transcript termination. An increase in beta-galactosidase mRNA and the virtual absence of full length endogenous mRNA confirmed gene disruption in mouse embryonic fibroblasts generated from embryonic day 13.5 homozygous mutant mice. However, variable but detectable expression of full length endogenous mRNA in tissues from adult mutant mice using primers specific for exon 3 confirmed that the insertion of the GT cassette resulted in a hypomorphic allele. (J:226674)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  KST256	CC156355 ; (MGI Seq Detail)	5' RACE	Chr12:44256722-44256851 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  3 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnajb9 Mutation: 13 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 5 reference(s)