Rfx2^{Gt(IST10638H11)Tigm}
Gene trapped Allele Detail

Summary

• Symbol: Rfx2^{Gt(IST10638H11)Tigm}
• Name: regulatory factor X, 2 (influences HLA class II expression); gene trap IST10638H11, Texas A&M Institute for Genomic Medicine
• MGI ID: MGI:3966900
• Synonyms: Rfx2^gt
• Gene: Rfx2 Location: Chr17:57082897-57138013 bp, - strand Genetic Position: Chr17, 29.5 cM, cytoband D
• Alliance: Rfx2^{Gt(IST10638H11)Tigm} page

In Rfx2^{Gt(IST10638H11)Tigm}/Rfx2^{Gt(IST10638H11)Tigm} testis, elongating spermatids are replaced by symblasts of spermatids

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: IST10638H11
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: Lex3.13 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: VICTR76 Vector Type: gene trap
• Mutation details: A lacZ reporter poly A gene cassette with a splice acceptor was inserted into intron 1 of the gene before the ATG start codon in exon 2. Transcription at the locus should result in the splicing of exon 1 to the gene trap vector and eliminate the production of the endogenous transcript. RT-PCR analysis of total RNA from E9.5 embryos confirmed the absence of detectable mRNA in homozygous mutant samples. X-gal staining revealed that lacZ activity was present in ciliated tissues during mouse development including the node, the floor plate and the dorsal neural tube. (J:227136)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  IST10638H11BBF1	EF768530 ; (MGI Seq Detail)	inverse PCR	Chr17:57129811-57130276 bp (+)
  IST10638H11HMF1	EF767431 ; (MGI Seq Detail)	inverse PCR	Chr17:57129811-57130014 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  85 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Schematic of the Rfx2^{Gt(IST10638H11)Tigm} allele

Expression

In Mice Carrying this Mutation:	24 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Rfx2 Mutation: 79 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)