Hic2^{Gt(E225A08)Wrst}
Gene trapped Allele Detail

Summary

• Symbol: Hic2^{Gt(E225A08)Wrst}
• Name: hypermethylated in cancer 2; gene trap E225A08, German Gene Trap Consortium
• MGI ID: MGI:3919233
• Synonyms: Hic^FL-U
• Gene: Hic2 Location: Chr16:17051451-17081294 bp, + strand Genetic Position: Chr16, 10.68 cM, cytoband B1
• Alliance: Hic2^{Gt(E225A08)Wrst} page

Mutation origin

• Mutant Cell Line: E225A08
• Germline Transmission: Earliest citation of germline transmission: J:225226
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: rsFROSAbgeo+2s
• Mutation details: The gene trap cassette is flanked by 2 pairs of variant LoxP sites (LoxP and Lox511) and 2 pairs of variant flippase recognition target sites (FRT and F3). The variant sites are designed to allow irreversible rotation of the genetrap cassette. Recombination between 2 variant sites in reverse orientation rotates the genetrap but leaves the other 2 variant sites in the same orientation on 1 side of the genetrap resulting in an excision event removing the possibility of a second rotation with the same enzyme. The genetrap can be recombined in this way twice, once with flippase (FLP) and once with CRE. This unrecombined allele expresses beta-galactosidase in place of exons 2 and 3. (J:225226)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  5SE225A08	FI575512 ; (MGI Seq Detail)	5'Splinkerette PCR	Chr16:17072719-17073368 bp (+)
  3SE225A08	FI575511 ; (MGI Seq Detail)	3'Splinkerette PCR	Chr16:17073404-17073705 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hic2 Mutation: 257 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)