Akap13^{Gt(CSJ306)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Akap13^{Gt(CSJ306)Byg}
• Name: A kinase anchor protein 13; gene trap CSJ306, BayGenomics
• MGI ID: MGI:3880669
• Synonyms: deltaGEF
• Gene: Akap13 Location: Chr7:75105282-75404357 bp, + strand Genetic Position: Chr7, 43.76 cM, cytoband D1
• Alliance: Akap13^{Gt(CSJ306)Byg} page

Increased fibrosis in Akap13^{Gt(CSJ306)Byg}/Akap13^{Gt(CSJ306)Byg} hearts in response to isoproterenol treatment

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: CSJ306
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The beta-geo gene trap was inserted into intron 23. This mutation creates a fusion of all three isoforms that disrupts the Rho-guanine nucleotide exchange factor (GEF) and protein kinase D (PKD) binding domains. Quantitative PCR analysis of neonatal heart and lung RNA revealed that expression of the E37-38 exon-exon junction is reduced in heterozygous and completely eliminated in homozygous mutant mice, while expression of the E4-5 exon-exon junction remains unaffected. (J:231257)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  CSJ306	EI183081 ; (MGI Seq Detail)	5' RACE	Chr7:75367514-75375112 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  59 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Akap13^{Gt(AG0213)Wtsi}, Akap13^{Gt(CSJ288)Byg}, and Akap13^{Gt(CSJ306)Byg} alleles

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Akap13 Mutation: 178 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)