Nsun2^{Gt(D014D11)Wrst}
Gene trapped Allele Detail

Summary

• Symbol: Nsun2^{Gt(D014D11)Wrst}
• Name: NOL1/NOP2/Sun domain family member 2; gene trap D014D11, German Gene Trap Consortium
• MGI ID: MGI:3873208
• Synonyms: Misu^-
• Gene: Nsun2 Location: Chr13:69760135-69783899 bp, + strand Genetic Position: Chr13, 35.55 cM
• Alliance: Nsun2^{Gt(D014D11)Wrst} page

Mutation origin

• Mutant Cell Line: D014D11
• Germline Transmission: Earliest citation of germline transmission: J:179810
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: rFlpROSAbetageo
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. Gene trap insertion is in intron 8 and resuls in disruption of the catalytic domain encoded be exons 2-12. Q-PCR demonstrated the absence of specific mRNA in homozygotes and Western blot analysis confirmed the absence of protein. (J:179810)
• Sequence Tags: Sequence tag details (3 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  3SD014D11	ER897017 ; (MGI Seq Detail)	3'Splinkerette PCR	Chr13:69770753-69771268 bp (+)
  D014D11	DU821397 ; (MGI Seq Detail)	5' RACE	Chr13:69766248-69769797 bp (+)
  5SD014D11	ER897018 ; (MGI Seq Detail)	5'Splinkerette PCR	Chr13:69770457-69770624 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  9 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nsun2 Mutation: 41 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 10 reference(s)