Lemd2^{Gt(DD0639)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Lemd2^{Gt(DD0639)Wtsi}
• Name: LEM domain containing 2; gene trap DD0639, Wellcome Trust Sanger Institute
• MGI ID: MGI:3871218
• Synonyms: Lemd2^gt
• Gene: Lemd2 Location: Chr17:27408574-27423443 bp, - strand Genetic Position: Chr17, 13.82 cM
• Alliance: Lemd2^{Gt(DD0639)Wtsi} page

Mutation origin

• Mutant Cell Line: DD0639
• Germline Transmission: Earliest citation of germline transmission: J:221495
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1lxrT2
• Mutation details: The gene trap introduced a sequence between exons 3 and 4 of the gene that contains a splice acceptor site (SA), a cDNA encoding the beta-galactosidase reporter linked to neomycin (geo), and a SV40 cleavage/polyadenylation site (pA). While the mutant allele is predicted to generate an ~175-kDa fusion protein, a full length expression product was not detected. Smaller presumptive degredation products were seen on Western blot, albeit at reduced levels. (J:221495)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  DD0639	DU636424 ; (MGI Seq Detail)	5' RACE	Chr17:27418336-27422890 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	93 assay results
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lemd2 Mutation: 22 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 2 reference(s)