Igh^{tm2.1(Tag)Rwhe}
Targeted Allele Detail

Summary

• Symbol: Igh^{tm2.1(Tag)Rwhe}
• Name: immunoglobulin heavy chain complex; targeted mutation 2.1, Rudolf Hendriks
• MGI ID: MGI:3850723
• Synonyms: IgH.TEmu
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150315
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A floxed neomycin resistance gene, a splice accepter, an internal ribosome entry site, a SV40 large and small T antigen coding region, and an extra copy of the IgH intronic enhancer Emu were inserted in a reverse orientation between DQ52 and JH1 in the IgH locus. The neomycin gene was removed after targeting by crossing founder mice with transgenic mice expressing cre in the germline. SV40 T antigen transcripts were detected by RT-PCR in B cells. (J:150315)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 45 strains or lines available

References

• Original: J:150315 ter Brugge PJ, et al., A mouse model for chronic lymphocytic leukemia based on expression of the SV40 large T antigen. Blood. 2009 Jul 2;114(1):119-27
• All: 5 reference(s)