Igs1^{tm1(CAG-Bgeo,-tdTomato/TEVP,-SV2B/GFP)Nat}
Targeted Allele Detail

Summary

• Symbol: Igs1^{tm1(CAG-Bgeo,-tdTomato/TEVP,-SV2B/GFP)Nat}
• Name: intergenic site 1; targeted mutation 1, Jeremy Nathans
• MGI ID: MGI:3849062
• Synonyms: Iis1^{tm1(CAG-Bgeo,-tdTomato/TEVP,-SV2B/GFP)Nat}, Ubb^tm1Nat, ZTEVP-SV2BGFP
• Gene: Igs1 Location: unknown Genetic Position: Chr11, Syntenic
• Alliance: Igs1^{tm1(CAG-Bgeo,-tdTomato/TEVP,-SV2B/GFP)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167667
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: The following elements were inserted 2 kb 5' of Ubb via homologous recombination: CMV enhancer/beta-actin promoter (CAG), loxP site, nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo), 3 stop transcription sites, loxP site, tdTomato/Tobacco etch virus protease (TEVP), a 6xMyc epitope, a TEVP cleavage site, synaptic vesicle protein 2B (SV2B), GFP, 3xHA (hemagluttinin) epitope, rabbit beta-globin 3' UTR. (J:167667)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs1 Mutation: 10 strains or lines available

References

• Original: J:167667 Nathans J, A conditional knock-in mutation in ubiquitin b (Ubb) expressing lacZ or tdTomato/Tobacco etch virus protease (TEVP). MGI Direct Data Submission. 2011
• All: 3 reference(s)