Pld2^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Pld2^{tm1(KOMP)Vlcg}
• Name: phospholipase D2; targeted mutation 1, Velocigene
• MGI ID: MGI:3847515
• Synonyms: Pld2^-
• Gene: Pld2 Location: Chr11:70430890-70448936 bp, + strand Genetic Position: Chr11, 42.99 cM
• Alliance: Pld2^{tm1(KOMP)Vlcg} page
• IMPC: Pld2 gene page

Mutation origin

• Mutant Cell Lines: 11541A-A11, 11541B-D1
• Germline Transmission: Earliest citation of germline transmission: J:216114
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 16757bp between positions 70431675-70448431 of Chromosome 11 (Build GRCm39). RT-PCR analysis revealed absence of mRNA transcripts in homozygous mutant platelets. Western blot analysis confirmed the absence of protein in mutant spleen and brain tissues. (J:136110, J:216114)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Pld2 Mutation: 48 strains or lines available

Notes

J: 216114 and, presumably, J:215283 describe mice created using the 11541B-D1 mutant ES cell line.

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 5 reference(s)