Mif^tm2Gfr
Targeted Allele Detail

Summary

• Symbol: Mif^tm2Gfr
• Name: macrophage migration inhibitory factor (glycosylation-inhibiting factor); targeted mutation 2, G Fingerle-Rowson
• MGI ID: MGI:3842525
• Synonyms: mif^P1G
• Gene: Mif Location: Chr10:75695187-75696074 bp, - strand Genetic Position: Chr10, 38.59 cM, cytoband C1
• Alliance: Mif^tm2Gfr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147772
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Nucleotide substitutions (CCT->GGC) results in the amino acid substitution of proline with glycine at position 1 (P1G). A self-excising floxed neo cassette was inserted downstream of exon 3 and successfully self-excised following germ line passage. Enzymatic activity of the protein is absent. (J:147772)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mif Mutation: 21 strains or lines available

References

• Original: J:147772 Fingerle-Rowson G, et al., A tautomerase-null macrophage migration-inhibitory factor (MIF) gene knock-in mouse model reveals that protein interactions and not enzymatic activity mediate MIF-dependent growth regulation. Mol Cell Biol. 2009 Apr;29(7):1922-32
• All: 3 reference(s)