Clec9a^tm1Crs
Targeted Allele Detail

Summary

• Symbol: Clec9a^tm1Crs
• Name: C-type lectin domain family 9, member a; targeted mutation 1 Caetano Reis e Sousa
• MGI ID: MGI:3841125
• Synonyms: Clec9a^gfp
• Gene: Clec9a Location: Chr6:129385825-129401726 bp, + strand Genetic Position: Chr6, 63.3 cM
• Alliance: Clec9a^tm1Crs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147472
• Parent Cell Line: S6B6 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A farnesylated form of EGFP was inserted in frame with the two first amino acids of the gene locus. Half of the exon 1 and the entire exon 2 were eliminated with transcription following EGFP terminated by a strong polyadenylation signal. A loxP flanked neomycin cassette was included downstream of EGFP for targeting purposes. Gene inactivation was confirmed by a lack of protein expression by homozygote splenic CD8alpha⁺ dendritic cells. GFP expression was detected in the same population of dendritic cells. (J:147472)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clec9a Mutation: 28 strains or lines available

Notes

The expression of NK1.1 (Klrb1c) is linked to Clec9a deficiency in homozygous mice, indicating that the homologous recombination step targeted the chromosome of C57BL/6J origin.

References

• Original: J:147472 Sancho D, et al., Identification of a dendritic cell receptor that couples sensing of necrosis to immunity. Nature. 2009 Apr 16;458(7240):899-903
• All: 2 reference(s)