Cdr2^tm1Rbd
Targeted Allele Detail

Summary

• Symbol: Cdr2^tm1Rbd
• Name: cerebellar degeneration-related 2; targeted mutation 1, Robert B Darnell
• MGI ID: MGI:3840617
• Synonyms: Cdr2^-
• Gene: Cdr2 Location: Chr7:120556259-120581535 bp, - strand Genetic Position: Chr7, 65.04 cM
• Alliance: Cdr2^tm1Rbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/Ola

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A vector was designed to replace the majority of the targeted gene's coding region - exon 2, all of exons 3 and 4, as well as the coding region in exon 5. EGFP was fused in-frame to the coding sequence within exon 2, followed by an FRT site, 1.3kb of the human glyceraldehyde-3-phosphate dehydrogenase (GAPD) 3'UTR and flanking sequence, a loxP-flanked neomycin cassette, and a second FRT site. The construct was electroporated into 129P2/Ola-derived embryonic stem (ES) cells. Correctly targeted ES cells were transfected with a cre-expressing plasmid to remove the neomycin cassette. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdr2 Mutation: 29 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 2 reference(s)