Sele^tm2Hyn
Targeted Allele Detail

Summary

• Symbol: Sele^tm2Hyn
• Name: selectin, endothelial cell; targeted mutation 2, Richard Hynes
• MGI ID: MGI:3839963
• Synonyms: E-selectin^-
• Gene: Sele Location: Chr1:163875773-163885246 bp, + strand Genetic Position: Chr1, 71.35 cM
• Alliance: Sele^tm2Hyn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:31626
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A modified D3 ES cell line heterozygous for the Selp^tm1Hyn allele was targeted a second time. The exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain were replaced by the insertion of a PGK-hygro cassette. Both Northern and RT-PCR analysis revealed an absence of normal mRNA for E-selectin but the presence of mRNA for P-selectin in extracts from cardiac and pulmonary tissue of homozygous mutant mice. This targeted mutation occurred in trans with the Selp^tm1Hyn mutation and were subsequently bred apart. (J:31626)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Sele Mutation: 52 strains or lines available

References

• Original: J:31626 Frenette PS, et al., Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell. 1996 Feb 23;84(4):563-74
• All: 15 reference(s)