Drinksac2^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Drinksac2^C57BL/6J
• Name: drink saccharin 2; C57BL/6J
• MGI ID: MGI:3838590
• QTL: Drinksac2 Location: Chr19:3695155-3695286 bp Genetic Position: Chr19, Syntenic

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Inheritance: Not Specified

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:144262

Linkage analysis was performed on (C57BL/6J x PWK/PhJ)F2 animals to identify genetic loci associated with preference and intake of calcium and other salt compounds. Animals were subjected to a two-bottle choice test between water and one other solution (CaCl2, CaLa, MgCl2, KCl, NH4Cl, NaCl, citric acid, QHCl, or saccharin) for a period of 96 hours. PWK/PhJ is a high consumer of calcium chloride (CaCl2) and calcium lactate (CaLa) whereas C57BL/6J is a relatively low consumer. PWK/PhJ also consumes more MgCl2 compared to C57BL/6J. However, when consumption of KCl, NaCl and saccharin were analyzed, C57BL/6J animals consumed more of these solutions compared to PWK/PhJ animals. Linkage analysis was performed using 116 polymorphic markers at a resolution of 10cM - 30cM. Thirty QTLs reaching statistical significance were identified. Authors assigned separate QTL nomenclature for each of the salt compound QTL even if they mapped to the same linkage marker.

Significant linkage to quinine monohydrochloride preference mapped to 4 Mb on mouse Chromosome 19 near D19Mit68 (LOD=5.8). This locus explains 5.5% of the variance in QHCl preference and is named Drinkqhcl2 (drink quinine monohydrochloride 2). C57BL/6J-derived alleles at Drinkqhcl2 confer increased preference for quinine monohydrochloride solution. D19Mit68 also displays significant linkage to saccharin intake with LOD=3.9. This locus is named Drinksac2 (drink saccharin 2). PWK/PhJ-derived alleles at Drinksac2 confer increased saccharin intake. Drinksac2 explains 4% of the variance for saccharin intake. Bits4 (15 cM; 20.42 Mb) is a previously identified bitterness QTL located near Drinksac2. Plcb3 (2.5 cM; 7.02 Mb) is a potential candidate gene for Drinksac2.

References

• Original: J:144262 Tordoff MG, et al., Calcium taste preferences: genetic analysis and genome screen of C57BL/6J x PWK/PhJ hybrid mice. Genes Brain Behav. 2008 Aug;7(6):618-28
• All: 1 reference(s)