Gt(ROSA)26Sor^{tm1(CAG-Kcnj11*,-GFP)Nich}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Kcnj11*,-GFP)Nich}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Colin G Nichols
• MGI ID: MGI:3838361
• Synonyms: mutKir6.2, Rosa26-Kir6.2, Rosa26-Kir6.2(K185Q,deltaN30)
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Kcnj11*,-GFP)Nich} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146650
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-Kcnj11*,-GFP)Nich} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Kcnj11 (MGI:107501)

• Mutation details: The targeting construct contained the CAG promoter (chicken beta actin promoter with CMV enhancers), a floxed neomycin-Westphal stop sequence (WSS) cassette, a modified Kcnj11 gene, an IRES and GFP sequence. Kcnj11 has been modified by a 30 amino acid N-terminal truncation and by a modification of the codon for amino acid residue 185 causing a lysine to glutamine substitution. Cre mediated excision of the neo-WSS cassette permits expression of the mutated Kcnj11 and GFP under the control of the CAG promoter. (J:146650)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1046 strains or lines available

References

• Original: J:146650 Remedi MS, et al., Secondary consequences of beta cell inexcitability: identification and prevention in a murine model of K(ATP)-induced neonatal diabetes mellitus. Cell Metab. 2009 Feb;9(2):140-51
• All: 11 reference(s)