Tg(Ins2-PTHLH)1807Rcv
Transgene Detail

Summary

• Symbol: Tg(Ins2-PTHLH)1807Rcv
• Name: transgene insertion 1807, Rupangi C Vasavada
• MGI ID: MGI:3837305
• Synonyms: RIP-PTHrP
• Transgene: Tg(Ins2-PTHLH)1807Rcv Location: unknown
• Alliance: Tg(Ins2-PTHLH)1807Rcv page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ins2-PTHLH)1807Rcv expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PTHLH (5744)

  Pthlh

  (MGI:97800)

• Tg(Ins2-PTHLH)1807Rcv expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Ins2 (24506)

  Ins2	(MGI:96573)

• Mutation details: The transgene construct contains a 650-bp segment of the promoter region of the rat insulin II gene that is truncated 30 bp upstream of the initiation codon on exon 1, which drives expression of a 568-bp EcoRI-StyI cDNA fragment of the human PTHLH(1-141) cDNA. This sequence contains the entire pre-pro and coding region sequences of the PTHLH(1-141) isoform but only 20 bp of 39 untranslated sequence. At the 3' end of the transgene construct, human growth hormone sequences containing transcription termination, polyadenylation, and splicing signals were added. RNAase protection assays and immunohistochemical staining confirmed the pancreatic overexpression of the human mRNA and protein, respectively. (J:30608)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:30608 Vasavada RC, et al., Overexpression of parathyroid hormone-related protein in the pancreatic islets of transgenic mice causes islet hyperplasia, hyperinsulinemia, and hypoglycemia. J Biol Chem. 1996 Jan 12;271(2):1200-8
• All: 3 reference(s)