Hprt1^{tm1(Pbsn*-cre/ERT2)Jir}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(Pbsn*-cre/ERT2)Jir}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Frank R Jirik
• MGI ID: MGI:3831277
• Synonyms: ARR2PB-CreER(T2)
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(Pbsn*-cre/ERT2)Jir} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144541
• Parent Cell Line: BK4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Hprt1^{tm1(Pbsn*-cre/ERT2)Jir} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Pbsn (54193)

  Pbsn	(MGI:1860484)

• Mutation details: A 500 bp composite promoter construct having two tandem androgen response elements fused to a short rat Probasin promoter fragment was placed upstream of a cre/ERT2 expression cassette. This construct was placed into a Hprt rescue vector (containing Hprt promoter sequence and the first three exons) and was used in recombination of ES cells containing a spontaneous deletion spanning the first two exons of Hprt. Clones undergoing homologous recombination were isolated by virtue of their resistance to HAT selection, which is mediated by Hprt expression. When founder mice were crossed to mice carrying cre-reporter alleles, cre expression was found to be specifically expressed in the prostrate epithelium and was strictly tamoxifen dependent. (J:144541)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pbsn (rat) Summary of all recombinase alleles driven by Pbsn. Alliance rat Pbsn gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1274 strains or lines available

References

• Original: J:144541 Luchman HA, et al., Temporally controlled prostate epithelium-specific gene alterations. Genesis. 2008 Apr;46(4):229-34
• All: 3 reference(s)