Itga4^tm1Mshi
Targeted Allele Detail

Summary

• Symbol: Itga4^tm1Mshi
• Name: integrin alpha 4; targeted mutation 1, Motomu Shimaoka
• MGI ID: MGI:3830623
• Synonyms: Itga4-R/A^GFFKR
• Gene: Itga4 Location: Chr2:79085770-79163467 bp, + strand Genetic Position: Chr2, 47.38 cM
• Alliance: Itga4^tm1Mshi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144092
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 28 was replaced with one in which nucleotide substitutions resulted in amino acid substitution of GFFKA for GFFKR. This sequence is involved in the formation of a membrane-proximal salt bridge necessary for inactivation. A self-excising neo cassette was inserted upstream of exon 28 and subsequently deleted in chimeras. (J:144092)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Itga4 Mutation: 62 strains or lines available

References

• Original: J:144092 Imai Y, et al., Genetic perturbation of the putative cytoplasmic membrane-proximal salt bridge aberrantly activates alpha(4) integrins. Blood. 2008 Dec 15;112(13):5007-15
• All: 2 reference(s)