Rap1a^tm1Tze
Targeted Allele Detail

Summary

• Symbol: Rap1a^tm1Tze
• Name: RAS-related protein 1a; targeted mutation 1, Tomasz Zemojtel
• MGI ID: MGI:3830343
• Gene: Rap1a Location: Chr3:105634583-105708652 bp, - strand Genetic Position: Chr3, 46.45 cM
• Alliance: Rap1a^tm1Tze page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144145
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct was made by replacing a 420-bp region encoding the N-terminal 19 amino acids, which include conserved residues and part of the GTP-binding motif, with an internal ribosome entry site (IRES)/LacZ/ neomycin cassette. Shortened mRNAs for the locus were detected in tissue of homozygous mice. These resulted from an alternative splicing site 5' of the neomycin cassette and an alternative ATG start site that would result in a 51 amino acid truncated protein. However, no protein was detected in immunoblot analysis of thymus and spleen extracts so the authors concluded this was a null allele. LacZ staining was evident in E12.5 embryos. (J:144145)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rap1a Mutation: 23 strains or lines available

References

• Original: J:144145 Duchniewicz M, et al., Rap1A-deficient T and B cells show impaired integrin-mediated cell adhesion. Mol Cell Biol. 2006 Jan;26(2):643-53
• All: 3 reference(s)