Il17f^{tm1(Thy1)Weav}
Targeted Allele Detail

Summary

• Symbol: Il17f^{tm1(Thy1)Weav}
• Name: interleukin 17F; targeted mutation 1, Casey T Weaver
• MGI ID: MGI:3830280
• Synonyms: Il17f^Thy1.1, IL-17F-Thy1.1 reporter
• Gene: Il17f Location: Chr1:20847370-20855498 bp, - strand Genetic Position: Chr1, 6.49 cM
• Alliance: Il17f^{tm1(Thy1)Weav} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:143729
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A reporter construct was made consisting of an IRES domain, coding sequence for a variant of murine Thy1 (Thy1.1), and a loxP-flanked neomycin cassette. The vector was targeted to exon 3 of the gene locus immediately downstream of the stop codon and within the 3' untranslated portion of the gene. Chimeric founders were bred with B6.EIIa-Cre transgenic mice to remove the neomycin selection cassette. CD4⁺ T cells isolated from homozygote mice and cultured under Th17 conditions co-expressed both IL-17F and Thy1.1 in a log-linear manner. (J:143729)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il17f Mutation: 27 strains or lines available

References

• Original: J:143729 Lee YK, et al., Late developmental plasticity in the T helper 17 lineage. Immunity. 2009 Jan;30(1):92-107
• All: 13 reference(s)