Tgq28^DBA/2J
QTL Variant Detail

Summary

• QTL variant: Tgq28^DBA/2J
• Name: triglyceride QTL 28; DBA/2J
• MGI ID: MGI:3828461
• QTL: Tgq28 Location: Chr16:79986646-79986646 bp Genetic Position: Chr16, Syntenic

Variant origin

• Strain of Specimen: DBA/1J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• Mutation details: This allele confers increased plasma triclygeride concentration in males on both CHOW and high fat diets compared to DBA/1J. (J:142770)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Tgq28 is male-specific.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:142770

Linkage analysis was performed using a backcross derived from closely related inbred strains DBA/1J and DBA/2J. The genetic composition of these 2 inbred strains differs by just 5.6% at the SNP level. A total of 294 mice from (DBA/1J x DBA/2J)F1 x DBA/1Jand (DBA/1J x DBA/2J)F1 x DBA/2J populations were screened for 6,949 polymorphic SNP markers. Backcross mice were placed on a high-fat diet at 8 weeks of age and phenotyped for plasma triglyceride and HDL concentrations at 8, 11 and 16 weeks of age. Parental strain DBA/2J displays significantly increased plasma triglyceride concentrations on a CHOW diet compared to parental strain DBA/1J. Male DBA/2J animals display significantly increased plasma triglyceride and HDL concentrations after an 8 week high fat diet compared to DBA/1J males.

Linkage to plasma triglyceride levels on a CHOW diet (LOD=3.5), 3 week high fat diet (LOD=5.5), and 8 week high fat diet (LOD=3.9) mapped to 40 cM on mouse Chromosome 16 at peak marker rs4210264 (80.19 Mb). This male-specific locus is named Tgq28 (triglyceride QTL 28). DBA/2J-derived alleles at Tgq28 confer increased plasma triglyceride concentration in males. [Table 2 and 3].

Significant linkage to plasma HDL cholesterol concentration on a CHOW diet mapped to 39 cM (LOD=3.9) and is named Hdlq65 (HDL QTL 65). This locus is also suggestive for plasma HDL concentration on a 3-week high fat diet. [Table 2].

References

• Original: J:142770 Stylianou IM, et al., Differences in DBA/1J and DBA/2J reveal lipid QTL genes. J Lipid Res. 2008 Nov;49(11):2402-13
• All: 1 reference(s)