Nfkb1^tm1Sley
Targeted Allele Detail

Summary

• Symbol: Nfkb1^tm1Sley
• Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105; targeted mutation 1, Steven C Ley
• MGI ID: MGI:3822895
• Synonyms: Nfkb1^S927A,S932A, Nfkb1^SSAA/SSAA
• Gene: Nfkb1 Location: Chr3:135290416-135397308 bp, - strand Genetic Position: Chr3, 62.82 cM
• Alliance: Nfkb1^tm1Sley page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142340
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting vector was designed to substitute alanine for serine at positions 927 and 933 (S927A, S932A). Targeting took place in PC3 ES cells in which Cre recombinase expression is restricted to haploid spermatids. A loxP-flanked neomycin resistance cassette was included upstream of exon 24 for targeting purposes and was subsequently removed by Cre mediated recombination upon transmission through male chimeric mice. This mutation was predicted to prevent phosphorylation of the gene product by IKK making it resistant to signal-induced degradation. (J:142340)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfkb1 Mutation: 99 strains or lines available

Notes

PC3 ES cells are homozygous for Tg(Prm-cre)70Og.

References

• Original: J:142340 Sriskantharajah S, et al., Proteolysis of NF-kappaB1 p105 is essential for T cell antigen receptor-induced proliferation. Nat Immunol. 2009 Jan;10(1):38-47
• All: 4 reference(s)