Cftr^tm1Cwr
Targeted Allele Detail

Summary

• Symbol: Cftr^tm1Cwr
• Name: cystic fibrosis transmembrane conductance regulator; targeted mutation 1, Case Western Reserve University
• MGI ID: MGI:3822760
• Synonyms: Cftr^fl10, Exon 11 conditional
• Gene: Cftr Location: Chr6:18170686-18322767 bp, + strand Genetic Position: Chr6, 8.1 cM, cytoband A3
• Alliance: Cftr^tm1Cwr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142723
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 11 (exon 10 in legacy nomenclature numbering) and a floxed neo cassette was inserted downstream of exon 11. Cre-mediated recombination was used to remove the neo cassette leaving exon 11 floxed. (J:142723)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cftr Mutation: 97 strains or lines available

References

• Original: J:142723 Hodges CA, et al., Generation of a conditional null allele for Cftr in mice. Genesis. 2008 Oct;46(10):546-52
• All: 14 reference(s)