Gt(ROSA)26Sor^{tm1(Hesx1)Jpmb}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Hesx1)Jpmb}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Juan Pedro Martinez Barbera
• MGI ID: MGI:3822482
• Synonyms: R26^Cond-Hesx1
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Hesx1)Jpmb} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:135132
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Hesx1)Jpmb} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hesx1 (MGI:96071)

• Mutation details: The Hesx1 coding sequence was cloned into a vector containing a floxed cassette with a PGK-Neo selection marker and a tpA transcriptional stop sequence. By homologous recombination, this construct was inserted into the Gt(ROSA)26Sor locus. The loxP site orientations were inverted to avoid translation interference due to the presence of an ATG within the loxP sites. Upon Cre-mediated excision of the selection cassette/stop sequence, Hesx1 is transcribed under control of the Gt(ROSA)26Sor promoter. (J:135132)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:135132 Andoniadou CL, et al., Lack of the murine homeobox gene Hesx1 leads to a posterior transformation of the anterior forebrain. Development. 2007 Apr;134(8):1499-508
• All: 1 reference(s)