About   Help   FAQ
Targeted Allele Detail
Symbol: Hesx1tm2Jpmb
Name: homeobox gene expressed in ES cells; targeted mutation 2, Juan Pedro Martinez Barbera
MGI ID: MGI:3822474
Synonyms: Hesx1I26T
Gene: Hesx1  Location: Chr14:26716375-26724286 bp, + strand  Genetic Position: Chr14, 16.09 cM, cytoband A3-B
Alliance: Hesx1tm2Jpmb page
Germline Transmission:  Earliest citation of germline transmission: J:142649
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Allele Type:    Targeted
Mutation:    Nucleotide substitutions
Mutation detailsThe coding sequence for wild-type Hesx1 was mutated by PCR so that codon 26 (isoleucine) was changed to encode a threonine residue (ATT->ACC). A loxP-flanked PGK-Neo selection cassette was inserted into the first intron of the mutated Hesx1 sequence in the targeting vector. Homologous recombination in CCE ES cells replaced the wild-type sequence with the mutant sequence in the targeting vector. F1 animals were crossed with Actin-beta-cre mice to excise the PGK-Neo cassette. (J:142649)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hesx1 Mutation:  9 strains or lines available
Original:  J:142649 Sajedi E, et al., Analysis of mouse models carrying the I26T and R160C substitutions in the transcriptional repressor HESX1 as models for septo-optic dysplasia and hypopituitarism. Dis Model Mech. 2008 Nov;1(4-5):241-54
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Copyright, and Privacy Statement
Send questions and comments to User Support.
last database update
MGI 6.22
The Jackson Laboratory