Zbtb7b^tm3Itan
Targeted Allele Detail

Summary

• Symbol: Zbtb7b^tm3Itan
• Name: zinc finger and BTB domain containing 7B; targeted mutation 3, Ichiro Taniuchi
• MGI ID: MGI:3817542
• Synonyms: ThPok^FH
• Gene: Zbtb7b Location: Chr3:89284953-89300976 bp, - strand Genetic Position: Chr3, 39.09 cM, cytoband A1-A2
• Alliance: Zbtb7b^tm3Itan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141142
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 was replaced by a modified version of exon 1 that encoded a "Flag-HA" epitope tag at the amino terminus of the gene product. A floxed neomycin cassette was also placed downstream of the exon. After targeting, the neo-cassette was removed by transient expression of cre recombinase. Protein expression was confirmed by immunoblot assays using anti-HA antibody. (J:141142)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zbtb7b Mutation: 24 strains or lines available

References

• Original: J:141142 Muroi S, et al., Cascading suppression of transcriptional silencers by ThPOK seals helper T cell fate. Nat Immunol. 2008 Oct;9(10):1113-21
• All: 2 reference(s)