Pcna^tm1.1Mdsc
Targeted Allele Detail

Summary

• Symbol: Pcna^tm1.1Mdsc
• Name: proliferating cell nuclear antigen; targeted mutation 1.1, Matthew D Scharff
• MGI ID: MGI:3817295
• Gene: Pcna Location: Chr2:132091206-132095100 bp, - strand Genetic Position: Chr2, 64.15 cM
• Alliance: Pcna^tm1.1Mdsc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141098
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP-site was inserted upstream of exon 2 and a floxed hygromycin cassette was placed downstream of exon 4. Chimeric mice were mated to a cre recombinase transgenic strain to delete exons 2-4. Genomic PCR confirmed that the correct targeting of the vector and subsequent exon excision took place. (J:141098)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcna Mutation: 16 strains or lines available

References

• Original: J:141098 Roa S, et al., Ubiquitylated PCNA plays a role in somatic hypermutation and class-switch recombination and is required for meiotic progression. Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16248-53
• All: 1 reference(s)