Tg(tetO-TGFB1*C223S*C225S)1Glk
Transgene Detail

Summary

• Symbol: Tg(tetO-TGFB1*C223S*C225S)1Glk
• Name: transgene insertion 1, Adam Glick
• MGI ID: MGI:3815091
• Synonyms: tetOTGFB1
• Transgene: Tg(tetO-TGFB1*C223S*C225S)1Glk Location: unknown
• Alliance: Tg(tetO-TGFB1*C223S*C225S)1Glk page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The porcine TGFbeta1 (TGFB1) cDNA containing a double mutation where cysteines at positions 223 and 225 converted to serines is driven by a heptameric tetO sequence and a CMV minimal promoter. The construct also contains a beta-globin intron. Founders were identified by Southern blotting. Mice express the porcine TGFB1 constitutively as a result of the two mutations. TGFB1 expression can be suppressed by crossing this line with a line expressing the tTA transactivator and maintaining pregnant females on a doxycycline-containing water supply. (J:70670)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:70670 Liu X, et al., Conditional epidermal expression of TGFbeta 1 blocks neonatal lethality but causes a reversible hyperplasia and alopecia. Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9139-44
• All: 7 reference(s)