Masp2^tm1Tefu
Targeted Allele Detail

Summary

• Symbol: Masp2^tm1Tefu
• Name: MBL associated serine protease 2; targeted mutation 1, Teizo Fujita
• MGI ID: MGI:3814550
• Synonyms: sMAP^-
• Gene: Masp2 Location: Chr4:148687011-148699956 bp, + strand Genetic Position: Chr4, 78.76 cM, cytoband E1
• Alliance: Masp2^tm1Tefu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140667
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Alternative splicing of this locus produces different protein products. One such protein called small MBL-associated protein (sMAP) has its C-terminal end encoded by exon 5. This exon is not believed to be used by the other alternative transcipts of this gene locus. A floxed neomycin-resistance cassette replaced exon 5 of the loci and an additional loxP site was inserted into the promoter region for future conditional targeting of the first 4 exons. Homozygous mice do not produce the sMAP transcript as determined by Northern blot and quantitative RT-PCR analysis on liver extract. However, two other alternative transcripts associated with this locus, H and L, had their expression reduced to 2% of wild-type. Immunoblotting of serum failed to detect any of the transcripts. (J:140667)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Masp2 Mutation: 34 strains or lines available

References

• Original: J:140667 Iwaki D, et al., Small mannose-binding lectin-associated protein plays a regulatory role in the lectin complement pathway. J Immunol. 2006 Dec 15;177(12):8626-32
• All: 5 reference(s)