Inpp5k^tm1Brd
Targeted Allele Detail

Summary

• Symbol: Inpp5k^tm1Brd
• Name: inositol polyphosphate 5-phosphatase K; targeted mutation 1, Allan Bradley
• MGI ID: MGI:3810936
• Synonyms: Pps^Brdm1
• Gene: Inpp5k Location: Chr11:75521814-75539697 bp, + strand Genetic Position: Chr11, 45.92 cM
• Alliance: Inpp5k^tm1Brd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139745
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Duplication, Insertion
• Inpp5k^tm1Brd involves 1 genes/genome features (Inpp5k) View all
• Mutation details: A vector isolated from a targeting vector library described in J:115976 was used to generate this null allele. The vector consisted of an upstream portion of the Hprt gene, a loxP site, and a neomycin resistance cassette followed by approximately 6.5 kb of the gene locus that contained exons 4 through 7. A 1,040-bp fragment of this genomic segment was removed by HpaI and BstBI restriction enzyme digestion. This vector was used in gap repair targeting where the genomic gap in the vector is repaired by the chromosomal DNA template, creating a duplication on the chromosome. In this allele exons 4-7 were duplicated with the partial Hprt gene, loxP site and the neo cassette in between the duplicated regions. This was predicted to cause a frameshift mutation and a null allele. Heterozygotes had half the expression of the gene in quadriceps muscle compared to wild-type mice, as determined by immunoblot assays. (J:115976, J:139745)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Inpp5k Mutation: 28 strains or lines available

References

• Original: J:139745 Ijuin T, et al., Increased insulin action in SKIP heterozygous knockout mice. Mol Cell Biol. 2008 Sep;28(17):5184-95
• All: 2 reference(s)