Stra8^tm1.1Nbg
Targeted Allele Detail

Summary

• Symbol: Stra8^tm1.1Nbg
• Name: stimulated by retinoic acid gene 8; targeted mutation 1.1, Norbert B Ghyselinck
• MGI ID: MGI:3810145
• Synonyms: Stra8^L-
• Gene: Stra8 Location: Chr6:34897098-34916279 bp, + strand Genetic Position: Chr6, 15.2 cM
• Alliance: Stra8^tm1.1Nbg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139646
• Parent Cell Line: P1 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2 and a floxed neo cassette was inserted downstream of exon 4. Transient cre expression in ES cells was used to remove the neo cassette and exons 2 through 4. The absence of protein expression as confirmed by western blot analysis on testis extracts. (J:139646)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stra8 Mutation: 35 strains or lines available

References

• Original: J:139646 Mark M, et al., STRA8-deficient spermatocytes initiate, but fail to complete, meiosis and undergo premature chromosome condensation. J Cell Sci. 2008 Oct 1;121(Pt 19):3233-42
• All: 3 reference(s)