Creb1^tm1Jsca
Targeted Allele Detail

Summary

• Symbol: Creb1^tm1Jsca
• Name: cAMP responsive element binding protein 1; targeted mutation 1, J Simon C Arthur
• MGI ID: MGI:3809580
• Synonyms: CREB DN, Creb^S133A
• Gene: Creb1 Location: Chr1:64571963-64643707 bp, + strand Genetic Position: Chr1, 32.74 cM, cytoband C1/C5
• Alliance: Creb1^tm1Jsca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139576
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Inserted expressed sequence) Mutation: Insertion Mutation details: A minigene consisting of wild-type exon 4 fused to the downstream exons of this gene locus was used in a targeting vector. The minigene and a neo-selection cassette were flanked with loxP sites and placed upstream of a mutated exon 4 sequence that substitutes the serine at amino acid 133 to an Alanine. This targeting vector was used to replace the endogenous exon 4 of the gene locus. In the absence of Cre expression, splicing between exon 3 and the minigene results in expression of a wild-type protein. Following expression of Cre-recombinase, the minigene is removed and splicing occurs on to the mutated exon 4 leading to transcription of the Ser133Ala mutant. (J:139576)

Generation of a Chd6 Exon 12 -/- mouse line.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Creb1 Mutation: 59 strains or lines available

References

• Original: J:139576 Ananieva O, et al., The kinases MSK1 and MSK2 act as negative regulators of Toll-like receptor signaling. Nat Immunol. 2008 Sep;9(9):1028-36
• All: 10 reference(s)