G0s2^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: G0s2^{tm1(KOMP)Vlcg}
• Name: G0/G1 switch gene 2; targeted mutation 1, Velocigene
• MGI ID: MGI:3808572
• Synonyms: G0S2^-, G0s2^tm1Vlcg
• Gene: G0s2 Location: Chr1:192954468-192955496 bp, - strand Genetic Position: Chr1, 97.69 cM
• Alliance: G0s2^{tm1(KOMP)Vlcg} page
• IMPC: G0s2 gene page

Mutation origin

• Mutant Cell Lines: 11287A-D11, 11287A-F11, 11287A-G1, 11287A-G11
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 329 bp between positions 192954751-192955079 of Chromosome 1 (Build GRCm39). The G0S2 coding region was replaced by a lacZ and loxP-flanked neomycin resistance gene cassette and the lacZ gene was fused in-frame at the G0S2 ATG start codon. Immunoblot analysis confirmed the absence of protein expression in gonadal fat tissue from homozygous mutant mice. (J:136110, J:225236)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any G0s2 Mutation: 10 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 8 reference(s)