Nrxn3^tm2Sud
Targeted Allele Detail

Summary

• Symbol: Nrxn3^tm2Sud
• Name: neurexin III; targeted mutation 2, Thomas C Sudhof
• MGI ID: MGI:3797484
• Synonyms: Nrxn3beta cKO
• Gene: Nrxn3 Location: Chr12:88689646-90301709 bp, + strand Genetic Position: Chr12, 42.94 cM
• Alliance: Nrxn3^tm2Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:224592
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into the 5' end of a modified exon 17, which contains an EGFP reporter gene followed by a FRT-flanked neomycin resistance cassette and a loxP site. Flp-mediated recombination removed the selection cassette and left the modified exon 17 floxed. The GFP insert does not affect the expression of neurexin 3 gene, but it is very difficult to detect GFP signals in the tissue by immunohistochemistry or direct fluorescence. (J:224592)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nrxn3 Mutation: 80 strains or lines available

References

• Original: J:224592 Anderson GR, et al., beta-Neurexins Control Neural Circuits by Regulating Synaptic Endocannabinoid Signaling. Cell. 2015 Jul 30;162(3):593-606
• All: 4 reference(s)