Scube1^tm1Rbya
Targeted Allele Detail

Summary

• Symbol: Scube1^tm1Rbya
• Name: signal peptide, CUB domain, EGF-like 1; targeted mutation 1, Ruey-Bing Yang
• MGI ID: MGI:3796593
• Synonyms: Scube1^deltacub
• Gene: Scube1 Location: Chr15:83489200-83609222 bp, - strand Genetic Position: Chr15, 39.4 cM
• Alliance: Scube1^tm1Rbya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:136534
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A puromycin resistance gene followed by the Hprt minigene with polyadenylation sequence was inserted downstream of exon 16. The insertion is accompanied by a duplication of exons 11 through 16 in addition to premature truncation of the coding region following the Hprt minigene thereby preventing translation of the exons encoding the CUB domain. Expression of the truncated fusion product was confirmed by western blot analysis on brain extracts. (J:136534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scube1 Mutation: 48 strains or lines available

References

• Original: J:136534 Tu CF, et al., Domain and functional analysis of a novel platelet-endothelial cell surface protein, SCUBE1. J Biol Chem. 2008 May 2;283(18):12478-88
• All: 1 reference(s)