Klrk1^tm1Dhr
Targeted Allele Detail

Summary

• Symbol: Klrk1^tm1Dhr
• Name: killer cell lectin-like receptor subfamily K, member 1; targeted mutation 1, David H Raulet
• MGI ID: MGI:3790957
• Synonyms: NKG2D^-
• Gene: Klrk1 Location: Chr6:129587286-129600827 bp, - strand Genetic Position: Chr6, 63.44 cM
• Alliance: Klrk1^tm1Dhr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134509
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1b through exon 6 were replaced by a floxed neomycin resistance cassette through homologous recombination. Mice carrying germline mutations were crossed with a cre transgenic mouse strain to remove the neomycin selection cassette, leaving a single loxP site behind. Gene inactivation was confirmed by a lack of protein staining on NK cells using flow cytometry. (J:134509)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Klrk1 Mutation: 24 strains or lines available

References

• Original: J:134509 Guerra N, et al., NKG2D-deficient mice are defective in tumor surveillance in models of spontaneous malignancy. Immunity. 2008 Apr;28(4):571-80
• All: 28 reference(s)