Tg(Epo-DTA)#Nal
Transgene Detail

Summary

• Symbol: Tg(Epo-DTA)#Nal
• Name: transgene insertion, Nancy A Lee
• MGI ID: MGI:3779020
• Synonyms: PHIL, Tg(Epx-DTA)#Nal
• Transgene: Tg(Epo-DTA)#Nal Location: unknown
• Alliance: Tg(Epo-DTA)#Nal page

Transgene origin

• Strain of Origin: (CBA/CaJ X C57BL/6J)F1 x C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Transgenic mice were created that contain a DNA fragment containing 3717 bp of sequence upstream from the transcription start site of the mouse Epx gene (formerly EPO) as well as the first 54 bp encoding the 5'-untranslated region of the Epx mRNA followed by the open reading frame encoding the Diphtheria Toxin A chain. The DTA expression construct was completed with the addition of a series of exons-introns derived from a 2.1kb fragment encoding a truncated portion of the human growth hormone gene downstream of the DTA open reading frame. Analysis of circulating leukocytes demonstrated that transgenic animals were devoid of eosinophils but otherwise have a full complement of hematopoietically derived cells. (J:92831)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:92831 Lee JJ, et al., Defining a link with asthma in mice congenitally deficient in eosinophils. Science. 2004 Sep 17;305(5691):1773-6
• All: 33 reference(s)