Prkd1^tm1Eno
Targeted Allele Detail

Summary

• Symbol: Prkd1^tm1Eno
• Name: protein kinase D1; targeted mutation 1, Eric N Olson
• MGI ID: MGI:3778381
• Synonyms: PKD1^flox, PKD1^lox
• Gene: Prkd1 Location: Chr12:50388014-50695881 bp, - strand Genetic Position: Chr12, 21.78 cM, cytoband C1
• Alliance: Prkd1^tm1Eno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132803
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 12 and a FRT-flanked neomycin resistance cassette was inserted downstream of exon 14. Mice were crossed with FLPe transgenic mice to remove the neomycin resistance cassette, leaving exons 12-14 flanked by loxP sites. Correct targeting was confirmed by genomic PCR. (J:132803)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prkd1 Mutation: 55 strains or lines available

References

• Original: J:132803 Fielitz J, et al., Requirement of protein kinase D1 for pathological cardiac remodeling. Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):3059-63
• All: 17 reference(s)