Ifi208^{Tg(Cspg4-cre)1Akik}
Transgenic Allele Detail

Summary

• Symbol: Ifi208^{Tg(Cspg4-cre)1Akik}
• Name: interferon activated gene 208; transgene insertion 1, Akiko Nishiyama
• MGI ID: MGI:3778062
• Synonyms: Cspg4(NG2)-Cre, ng2-Cre, NG2creBAC, Tg(Cspg4-cre)1Akik
• Gene: Ifi208 Location: Chr1:173501241-173525961 bp, + strand Genetic Position: Chr1, 80.57 cM
• Alliance: Ifi208^{Tg(Cspg4-cre)1Akik} page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Ifi208^{Tg(Cspg4-cre)1Akik} expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cspg4 (MGI:2153093)

• Mutation details: The transgene construct consisted of the 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), which was modified by inserting an nls-cre coding sequence followed by a rabbit beta-globin polyA signal into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2 gene and to allow translation from the first ATG in the cre sequence. Cre expression is detected only in Cspg4-expressing cells at P1, P14 and P60 and not in Gfap +ve astrocytes or APC +ve oligodendrocytes. Activity is also detected in male germ cells. Line 1 inserted into an intron of the gene at 173692115 (Build GRCm38/mm10). Founder line 1 has a copy number of 1-3. (J:129201, J:185534)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cspg4 (mouse) Summary of all recombinase alleles driven by Cspg4. Comparative matrix view of recombinase activities Alliance mouse Cspg4 gene page

Tumor Data

List all tumor models in MMHCdb carrying Ifi208^{Tg(Cspg4-cre)1Akik}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ifi208 Mutation: 36 strains or lines available

References

• Original: J:129201 Zhu X, et al., NG2 cells generate both oligodendrocytes and gray matter astrocytes. Development. 2008 Jan;135(1):145-57
• All: 63 reference(s)